利用混池重测序鉴定萨福克绵羊超数排卵关键调控基因

doi:10.11843/j.issn.0366-6964.2019.09.008

Abstract

Abstract: The aim of this study was to identify the key regulatory genes affecting superovulation traits by genome pool resequencing, based on the superovulation treatment on Suffolk sheep. A total of 93 Suffolk donor sheep, aged 2-3 years old with good health conditions, were treated with superovulation. Thirty individuals with good superovulation result (the number of embryos obtained was greater than or equal to 15) were selected to construct a high-yield group. At the same time, another thirty individuals with poor superovulation result (the number of embryos obtained was less than or equal to 9) were selected to construct a low-yield group. After the genome pool resequencing, the SNPs identified in the two groups were analyzed by the selective sweep and gene enrichment. The results indicated that the average total embryos for each individual obtained in the superovulation were (12.55±7.97), and the average viable embryos for each individual were (7.76±7.43). The average viable embryos for the constructed high-yield and low-yield groups were (14.97±8.38) and (2.27±2.10), respectively. A total of 20 189 224 and 20 396 751 SNPs were detected from the two groups by resequencing. After the selective sweep and gene enrichment analysis, a total of 11 candidate genes identified could affect the superovulation of Suffolk sheep, in which, seven genes belong to the HOXA family, the function of two genes have not been verified, and the other two are DPH6 and AKAP6. In general, based on the genome pool resequencing of the high-yield and low-yield groups in superovulation of Suffolk sheep, a total of 11 key regulatory genes were identified. Further validation of the role of HOXA gene family, DPH6 and AKAP6 genes in follicular development may be important to explain the regulation mechanism of superovulation in livestock.

CLC Number:

S826.3

HUA Liushuai, XIN Xiaoling, WANG Jing, LUO Shengjin, ZENG Tao, BA Tima, SHI Qiaoting, XU Zhaoxue, WANG Eryao. Identification of Key Regulatory Genes for Superovulation in Suffolk Sheep by Pool Resequencing[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1813-1821.

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Identification of Key Regulatory Genes for Superovulation in Suffolk Sheep by Pool Resequencing

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